beet lab :: essays research papers

MATERIAL AND METHODS constancy of cell membrane using pHFor the lab experiment for examen the stability of common common beet cell membranes using pH, many materials were used as follows. Obtaining a beet we punch out cores, using a bobtail borer. After washing the cores we put severally one inside a start out streak tube, and added a different pH solution in each one. After 3 minutes in these pic solutions, we took the beet out with a dissecting needle. Then transferred each beet to a screen out test tube containing deionized water. After 20 minutes in these dispersal solutions, we took the beets out with a dissecting needle and discard it. We therefore stirred each solution in the test tube with a stirring rod, and transferred it to a cuvette. A spectrophotometer was then calibrated, and used to measure the absorbance of each exposure solution, and distribution solution. Membrane DamageFor the lab experiment for Membrane Damage, we tested the sublimate pigment and dil uted it. When the pH solutions are added, this will cause it to be in a range of absorbance. We used materials as follows. Obtaining a beet we proceeded to cut small individual blockages. We then rinse each cube to remove any damaged pigments with deionized water. Using a blender, we blend the beets with 15 mL of pH 7 DI water. After blending we used cheesecloth to separate the liquid from the solids for easier centrifuge process. Then we put the liquid beet into a centrifuge tube and centrifuge it for 5 minutes at 2500 rpm. We then remove the supernatant into a beaker, and discarded the sediment. Using a 14 ratio mixture of the supernatant and deionized water, we made a form solution. We then tested the stock solutions absorbance with a spectrophotometer, and mall 1 mL of the solution into separate test tubes. Next we added an spare 4 mL of pH solutions in the 2-11 range into each test tube. After mixing, we tested the absorbance for each solution using a spectrophotometer. co alesce the Diffusion and Exposure SolutionFor the lab experiment of mixing the diffusion and exposure solution, we are going to test if the pigment is released in the exposure solution. We used materials as follows Obtaining a beet, we punched out 2 cm tenacious beets using a cork borer. Then we wash the beets in trail water, after that we prepared 10 test tubes each containing different pH solution.