Apoptosis Induction

APOPTOSIS INDUCTION THROUGH DEATH RECEPTOR 5 AND CASPASE 3 ACTIVITY RECOGNITION BY IMMUNOCYTOCHEMISTRY

INTRODUCTION
cellphone polish
Cell culture is the process of growing cells invitro [microbial, plant or animal] chthonian specific environmental conditions by providing specific nutrients & developing factors. Each cell in the culture extracted from tissue is open of dividing [like micro organism] by mitosis into a cell population and ceases to proliferate when a parameter like nutrient depletion prevails or it depends on cell type, species of origin and age related changes referred to as Hayflick snare. Same types of cells are present in a culture [e.g. hepatocytes] and are genetically alike but sometimes picture genetic variation. (Butler 2005). The cells obtained from the source tissue by enzymatic, chemical or mechanical disintegration are called the base culture. When the primary culture is sub cultured, cell lines are obtained. The cells washstand be cultured as a monolayer culture or dangling culture. When a large number of cells are available, they can be stored for longer duration with the avail of cryopreservation, where the cells are stored under -120oC in cryopreservants like DMSO [dimethyl sulfoxide] and glycerol.



Cell cultures help us to case process of cell growth, differentiation, proliferation and other key inter and intracellular processes. Many key mechanisms occurring in cells have been studied with the help of cell cultures.
MTT assay
MTT (3-(4, 5 -Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole) assay is a colorimetric assay used for measuring enzyme activity. In this assay, MTT is decrease by the enzymes in the cell to formazan dyes, which gives out a majestic colour. The enzymes within the cells that reduce MTT and other tetrazolium salts to water insoluble color formazan crystals are the enzymes of respiratory chain and electron transport systems. The spectrophotometric or colorimetric data of the crystals...If you want to get a overflowing essay, order it on our website: Ordercustompaper.com



If you want to get a full essay, wisit our page: write my paper